abstract：:We have shown that in fixed mitotic chromosomes from female G. gerbillus cells the inactive X chromosome is distinctly less sensitive to DNAase I than the active X chromosome, as demonstrated by in situ nick translation. These results indicated that the specific chromatin conformation that renders potentially active g...

journal_title：Cell

authors： Kerem BS,Goitein R,Diamond G,Cedar H,Marcus M

更新日期：1984-09-01 00:00:00

abstract：:The human epsilon-globin gene has a number of alternative transcription-initiation sites located upstream of the canonical mRNA cap site. In three nonerythroid cell lines, "leaky" epsilon-globin transcription occurs exclusively from one of these upstream sites, the -200 cap site. Using a transient expression assay, we...

journal_title：Cell

authors： Allan M,Zhu JD,Montague P,Paul J

更新日期：1984-09-01 00:00:00

abstract：:We describe the isolation and characterization of two unusual amber suppressor forms of T4 tRNALeu. The sequences of the suppressor tRNAs can be described as hybrids of wild-type tRNALeu and suppressor tRNAGln molecules: the chain lengths and majority of the nucleotide residues corresponded to tRNALeu, but CUA anticod...

journal_title：Cell

authors： McClain WH,Foss K

更新日期：1984-08-01 00:00:00

abstract：:The rate-limiting enzyme of cholesterol biosynthesis, HMG CoA reductase, is controlled by negative feedback regulation of transcription. We have isolated the reductase gene from a bacteriophage lambda genomic library prepared from hamster UT-1 cells. The 25 kilobase gene is split into 20 exons. The 5' untranslated and...

journal_title：Cell

authors： Reynolds GA,Basu SK,Osborne TF,Chin DJ,Gil G,Brown MS,Goldstein JL,Luskey KL

更新日期：1984-08-01 00:00:00

abstract：:We have analyzed two mutations of the white-eye gene, which arose in flies subject to I-R hybrid dysgenesis. These mutations are associated with insertions of apparently identical 5.4 kb sequences, which we have cloned. We believe that these insertions are copies of the I factor controlling I-R hybrid dysgenesis. The ...

journal_title：Cell

authors： Bucheton A,Paro R,Sang HM,Pelisson A,Finnegan DJ

更新日期：1984-08-01 00:00:00

abstract：:The thymus exports a selected subset of virgin T lymphocytes to the peripheral lymphoid organs. The mature phenotype of these thymus emigrants is similar to that of medullary thymocytes and has been cited as supporting a medullary rather than cortical exit site. Using the monoclonal antibody MEL-14, we identify a 1%-3...

journal_title：Cell

authors： Reichert RA,Gallatin WM,Butcher EC,Weissman IL

更新日期：1984-08-01 00:00:00

abstract：:We constructed rabbit beta-globin genes with deletions in the large intron, extending from the midpoint toward the 5' or 3' splice sites. Analysis of transcripts in transformed HeLa cells showed that six 5' proximal intron nucleotides allowed normal splicing. Correct splicing at the 3' splice site required 12 or more ...

journal_title：Cell

authors： Wieringa B,Hofer E,Weissmann C

更新日期：1984-07-01 00:00:00

abstract：:Mitochondrial DNA from bovine tissue contains heterogeneous sequences located within an evolutionary conserved cytosine homopolymer sequence near the 5' end of the D-loop region. This part of the mammalian mitochondrial genome is known to contain the origin of heavy strand DNA synthesis and the major transcriptional p...

journal_title：Cell

authors： Hauswirth WW,Van de Walle MJ,Laipis PJ,Olivo PD

更新日期：1984-07-01 00:00:00

abstract：:Transposon mutagenesis and complementation analysis of the photosynthesis genes in Rhodopseudomonas capsulata is presented utilizing Tn5.7 mutagenized R-primes. The R-prime pRPS404 contains many of the genes necessary for the differentiation of the photosynthetic apparatus. Utilizing homologous recombination, 30 indep...

journal_title：Cell

authors： Zsebo KM,Hearst JE

更新日期：1984-07-01 00:00:00

abstract：:We have constructed a gene fusion using the promoter of Drosophila hsp70 and the structural gene for Drosophila alcohol dehydrogenase (Adh) and used this construct to transform Adh-deficient flies. In these transformants, Adh is expressed only after heat shock. Like hsp70 itself, this heat-shock-inducible Adh (Adhhs) ...

journal_title：Cell

authors： Bonner JJ,Parks C,Parker-Thornburg J,Mortin MA,Pelham HR

更新日期：1984-07-01 00:00:00

abstract：:We have determined the nucleotide sequence of the transposable maize controlling element Activator (Ac). The Ac element is 4563 bp long and has an imperfect terminal repetition of 11 bp. The element contains two open reading frames (ORF) encoding polypeptides of 839 and 210 amino acids. Evidence derived from structura...

journal_title：Cell

authors： Pohlman RF,Fedoroff NV,Messing J

更新日期：1984-06-01 00:00:00

abstract：:X-ray studies have established that the structure of a right-handed, Watson-Crick double helix can change from place to place along its length as a function of base sequence. The base pairs transmit deformations out to the phosphate backbone, where they can then be recognized by proteins and other DNA-binding reagents...

journal_title：Cell

authors： Drew HR,Travers AA

更新日期：1984-06-01 00:00:00

abstract：:Four RNA products have been characterized from a soluble system that accurately splices purified substrate RNA. The labeled substrate RNA contained the first and second exons of the major late transcription unit of adenovirus 2 and a shortened form of the first intervening sequence. One of these RNA products was the e...

journal_title：Cell

authors： Grabowski PJ,Padgett RA,Sharp PA

更新日期：1984-06-01 00:00:00

abstract：:We have shown that covalent conjugation of ubiquitin to proteins is temperature-sensitive in the mouse cell cycle mutant ts85 due to a specifically thermolabile ubiquitin-activating enzyme (accompanying paper). We show here that degradation of short-lived proteins is also temperature sensitive in ts85 , in contrast to...

journal_title：Cell

authors： Ciechanover A,Finley D,Varshavsky A

更新日期：1984-05-01 00:00:00

abstract：:Using double-label quantitative immunoelectron microscopy on ultrathin cryosections of rat liver, we have compared the endocytotic pathways of the receptors for asialoglycoprotein (ASGP-R), mannose-6-phosphate ligands (MP-R), and polymeric IgA (IgA-R). All three were found within the Golgi complex, along the entire pl...

journal_title：Cell

authors： Geuze HJ,Slot JW,Strous GJ,Peppard J,von Figura K,Hasilik A,Schwartz AL

更新日期：1984-05-01 00:00:00

abstract：:Pulsed field gradient gel electrophoresis fractionates chromosome-sized DNA molecules from T. brucei. About 60% of the DNA remains in or close to the gel slot (large DNA). There are about three chromosomes of approximately 2 Mb, at least six chromosomes of 200-700 kb, and roughly a hundred mini-chromosomes of 50-150 k...

journal_title：Cell

authors： Van der Ploeg LH,Schwartz DC,Cantor CR,Borst P

更新日期：1984-05-01 00:00:00

abstract：:Transplantation of pronuclei between one-cell-stage embryos was used to construct diploid mouse embryos with two female pronuclei ( biparental gynogenones ) or two male pronuclei ( biparental androgenones ). The ability of these embryos to develop to term was compared with control nuclear-transplant embryos in which t...

journal_title：Cell

authors： McGrath J,Solter D

更新日期：1984-05-01 00:00:00

abstract：:Inducer T-cell clones reactive to the p-azobenzenearsonate (arsonate) hapten possess binding sites for radioactive arsanylated proteins, which are not present on clones with other antigen specificities. Binding occurred in the absence of histocompatibility proteins. Binding was specific for the p-azobenzenearsonate ha...

journal_title：Cell

authors： Rao A,Ko WW,Faas SJ,Cantor H

更新日期：1984-04-01 00:00:00

abstract：:The v-erbB gene of avian erythroblastosis virus (AEV) codes for an integral plasma membrane glycoprotein, gp74erbB. Expression of gp74erbB and its intracellular precursors, gp66erbB and gp68erbB, has been studied in cells transformed by two temperature-sensitive mutants of AEV. After shift to 42 degrees C, the process...

journal_title：Cell

authors： Beug H,Hayman MJ

更新日期：1984-04-01 00:00:00

abstract：:Plasmids containing the origin region of human mtDNA are specifically transcribed by the partially purified homologous mtRNA polymerase in vitro. Transcription of both the light and heavy strands initiates at the same sites previously identified as in vivo transcription start sites. The sequences responsible for initi...

journal_title：Cell

authors： Bogenhagen DF,Applegate EF,Yoza BK

更新日期：1984-04-01 00:00:00

abstract：:The globin and immunoglobulin multigene families have been used to study the effect of chromosomal organization on the time of gene replication. Some of the genes are late-replicating, providing the first identification of late-replicating sequences that are not highly repetitive. One is a member of the mouse alpha-gl...

journal_title：Cell

authors： Calza RE,Eckhardt LA,DelGiudice T,Schildkraut CL

更新日期：1984-03-01 00:00:00

abstract：:The major site of in vivo transcriptional initiation for both heavy and light strands of human mitochondrial DNA is the displacement-loop region. Transcripts synthesized in vitro by human mitochondrial RNA polymerase were mapped to the nucleotide level and have identical 5' end map positions to those reported for in v...

journal_title：Cell

authors： Chang DD,Clayton DA

更新日期：1984-03-01 00:00:00

abstract：:Our previous deletion experiments have shown that a short region of yeast nuclear gene introns containing the conserved sequence 5'-TACTAACA-3' is essential for splicing. In this report we show that the chemically synthesized decanucleotide 5'-TGTACTAACA-3', when introduced into a hybrid gene forming unspliceable RNA ...

journal_title：Cell

authors： Langford CJ,Klinz FJ,Donath C,Gallwitz D

更新日期：1984-03-01 00:00:00

abstract：:We have transferred the mouse Ak alpha and Ak beta genes, which encode the class II I-Ak molecule, into mouse L-cell fibroblasts and hamster B cells. I-Ak molecules are expressed on the surface of both cell types. The L-cell and hamster B-cell I-Ak molecules appear normal by serological analyses and two-dimensional ge...

journal_title：Cell

authors： Malissen B,Price MP,Goverman JM,McMillan M,White J,Kappler J,Marrack P,Pierres A,Pierres M,Hood L

更新日期：1984-02-01 00:00:00

abstract：:The single, chloroplast encoded gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) from Euglena gracilis is found to contain nine intervening sequences. The intervening sequences were identified by heteroduplex analysis between Euglena rbcL and the non-intron-containing rbcL from Spin...

journal_title：Cell

authors： Koller B,Gingrich JC,Stiegler GL,Farley MA,Delius H,Hallick RB

更新日期：1984-02-01 00:00:00

abstract：:Bovine Papilloma Virus (BPV-1) establishes itself as a multicopy nuclear plasmid in somatic mammalian cells in culture. We report here that two discontinuous regions within the viral genome can independently support extrachromosomal replication of the Tn5 neomycinr gene in cells that provide viral factors in trans. Th...

journal_title：Cell

authors： Lusky M,Botchan MR

更新日期：1984-02-01 00:00:00

abstract：:DNA sequence analysis of a class I gene (Q10), which maps to the Qa2,3 locus in the C57BL/10 (H-2b haplotype) mouse, reveals that it is almost identical to a cDNA clone (pH16) isolated from a SWR/J (H-2q haplotype) mouse liver cDNA library. Exon 5, in particular, has an unusual structure such that a polypeptide produc...

journal_title：Cell

authors： Mellor AL,Weiss EH,Kress M,Jay G,Flavell RA

更新日期：1984-01-01 00:00:00

abstract：:Unique loci in the human genome were examined with restriction enzymes in order to detect restriction fragment length polymorphisms (RFLPs). Of 31 arbitrary loci, nine were detectably polymorphic, reflecting ten polymorphic restriction sites. Nine of the ten polymorphic sites were revealed with two restriction enzymes...

journal_title：Cell

authors： Barker D,Schafer M,White R

更新日期：1984-01-01 00:00:00

abstract：:Release of CHO-K1 cells from plateau or stationary phase and reentry into the cell cycle is specifically and reversibly blocked at two distinct sites by the anticalmodulin drug W13. The first block occurs early during release while the cells are still at G0/G1, whereas the second occurs later in reentry during early S...

journal_title：Cell

authors： Chafouleas JG,Lagacé L,Bolton WE,Boyd AE 3rd,Means AR

更新日期：1984-01-01 00:00:00

abstract：:NRK fibroblasts exposed to transforming growth factor-beta (TGF-beta) show increased binding of radiolabeled epidermal growth factor (EGF) relative to untreated cells. The binding of another growth factor, rat insulin-like growth factor-II, is unaffected. The increase in EGF binding induced by TGF-beta is not due to i...

journal_title：Cell

authors： Assoian RK,Frolik CA,Roberts AB,Miller DM,Sporn MB

更新日期：1984-01-01 00:00:00

abstract：:We have localized ten oxi3- mutations in the first, al1, intron of the coxl gene. All are splicing deficient, being unable to excise the intron. Complementation experiments disclose several domains in the intron al1: the 5'-proximal and 3'-proximal domains harbor cis-dominant mutations, while trans-recessive ones are ...

journal_title：Cell

authors： Carignani G,Groudinsky O,Frezza D,Schiavon E,Bergantino E,Slonimski PP

更新日期：1983-12-01 00:00:00

abstract：:We have examined the conformation of DNA cloned from the hypersensitive region 5' of the chicken adult beta-globin gene, in order to determine whether the unusual sensitivity to nucleases is related to altered secondary structure of the DNA. The region contains a large number of sites for methylation; we have studied ...

journal_title：Cell

authors： Nickol JM,Felsenfeld G

更新日期：1983-12-01 00:00:00

abstract：:The monoclonal antibody ECCD-1 inhibits Ca2+-dependent cell-cell adhesion in teratocarcinoma cells, recognizing a cell surface component of MW 124,000. When mouse embryos at various preimplantation stages were cultured in the presence of ECCD-1, the compacted morphology of the 8- to 16-cell-stage embryos was destroyed...

journal_title：Cell

authors： Shirayoshi Y,Okada TS,Takeichi M

更新日期：1983-12-01 00:00:00

abstract：:Embryonal carcinoma cell lines present strong developmental analogies with early embryonic cells. Previous studies have shown that treatment of F9 teratocarcinoma cells with retinoic acid induces the expression of the classical transplantation antigens which are indispensable for effective interactions between cells. ...

journal_title：Cell

authors： Tanaka K,Appella E,Jay G

更新日期：1983-12-01 00:00:00

abstract：:We have developed an in vitro system utilizing yeast cell-free extracts to catalyze recombination events between homologous plasmids containing different mutant alleles of the Tet or ARG4 genes. The reaction increased the frequency of Tcr or Arg+ transformants (recombinants) from 2 X 10(-6) to 1-3 X 10(-3). Linearizin...

journal_title：Cell

authors： Symington LS,Fogarty LM,Kolodner R

更新日期：1983-12-01 00:00:00

abstract：:Fractionation of HeLa cell extracts reveals the presence of a promoter-specific transcription factor, Sp 1, which activates a class of promoters that includes the SV40 early promoter but not several others that have been tested. We analyzed SV40 early-promoter deletion mutants and determined that transcriptional activ...

journal_title：Cell

authors： Dynan WS,Tjian R

更新日期：1983-11-01 00:00:00

abstract：:When the human beta-globin gene is transfected into Hela cells, no beta-globin RNA is detected unless the gene is linked to a viral transcription enhancer. In this paper we show that trans-acting adenovirus and herpesvirus (pseudorabies) transcriptional regulatory proteins can circumvent this enhancer requirement for ...

journal_title：Cell

authors： Green MR,Treisman R,Maniatis T

更新日期：1983-11-01 00:00:00

abstract：:A mutation in any one of eight spoO genes of Bacillus subtilis blocks the process of spore formation at its earliest stage. To investigate how the products of the spoO genes may be involved in developmental gene expression, we fused the lacZ gene of E. coli to spoVG, a sporulation gene whose induction at the onset of ...

journal_title：Cell

authors： Zuber P,Losick R

更新日期：1983-11-01 00:00:00

abstract：:Properties of human anticentromere autoantibodies were analyzed. In intact cells or isolated cell fractions, these sera stain the centromeres of mitotic chromosomes and discrete speckles (prekinetochores) in nuclei. Staining is also retained in matrix preparations from nuclei or chromosomes. Immunoprecipitation or imm...

journal_title：Cell

authors： Cox JV,Schenk EA,Olmsted JB

更新日期：1983-11-01 00:00:00

abstract：:The rate of in vivo transcription from the E. coli tRNA and rRNA promoters depends on both cellular growth rate and aminoacid availability. To investigate the molecular mechanisms involved we determined the extent of interaction of RNA polymerase with the promoter of the tyrT stable RNA gene. We show that the enzyme c...

journal_title：Cell

authors： Travers AA,Lamond AI,Mace HA,Berman ML

更新日期：1983-11-01 00:00:00